Human CRP ELISA Kit
€0.00
In stock
SKU
BEK1367
Catalog Number: BEK1367
Size(s): 96 tests/kit
Standard Range: 780 pg/ml - 50,000 pg/ml
Sensitivity: <34 pg/ml
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Size(s): 96 tests/kit
Standard Range: 780 pg/ml - 50,000 pg/ml
Sensitivity: <34 pg/ml
Request Information
Introduction:
C-reactive protein (CRP) is an annular (ring-shaped) pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q.CRP is synthesized by the liverin response to factors released by macrophages, T cells and fat cells (adipocytes).It is a member of the pentraxin family of proteins.It is not related to C-peptide (insulin) or protein C (blood coagulation). C-reactive protein was the first pattern recognition receptor (PRR) to be identified.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CRP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CRP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.
Application:
For quantitative detection of CRP in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
C-reactive protein (CRP) is an annular (ring-shaped) pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q.CRP is synthesized by the liverin response to factors released by macrophages, T cells and fat cells (adipocytes).It is a member of the pentraxin family of proteins.It is not related to C-peptide (insulin) or protein C (blood coagulation). C-reactive protein was the first pattern recognition receptor (PRR) to be identified.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CRP polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CRP polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.
Application:
For quantitative detection of CRP in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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