Human CSN1 (Casein Alpha) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Alpha(CSN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Alpha(CSN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Alpha(CSN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Alpha(CSN1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
CASa, CSN1, CSN1S1, Casein Alpha S1, Caseinate, Caseine/caséine/Kasein/caseína, Alpha-S1-casein, Casoxin-D

Sample Type:
tissue homogenates, cell lysates, cell culture supernates, breast milk and other biological fluids

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only