Human CTACK (Cutaneous T-Cell Attracting Chemokine) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cutaneous T-Cell Attracting Chemokine(CTACK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cutaneous T-Cell Attracting Chemokine(CTACK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cutaneous T-Cell Attracting Chemokine(CTACK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cutaneous T-Cell Attracting Chemokine(CTACK) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Tumor immunity, Infection immunity, Dermatology

Alternative Names:
CCL27, ILC, SCYA27, ALP, Skinkine, ESkine, PESKY, CTAK, Chemokine(C-C-Motif)ligand 27, Small Inducible Cytokine Subfamily A Member 27, IL-11 R-alpha-locus chemokine

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only