Human CTAG1B (Cancer/Testis Antigen 1B) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cancer/Testis Antigen 1B(CTAG1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cancer/Testis Antigen 1B(CTAG1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cancer/Testis Antigen 1B(CTAG1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cancer/Testis Antigen 1B(CTAG1B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Tumor immunity

Alternative Names:
CTAG, CTAG1, ESO1, LAGE-2, LAGE2B, NY-ESO-1, CAMEL, ESO2, LAGE-1, LAGE-2b, LAGE2A, Autoimmunogenic cancer/testis antigen NY-ESO-1, L antigen family member 2

Sample Type:
serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only