Human CYP2C8 (Cytochrome P450 2C8) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP2C8(Cytochrome P450 2C8). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CYP2C8(Cytochrome P450 2C8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP2C8(Cytochrome P450 2C8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CYP2C8(Cytochrome P450 2C8) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Infection immunity,

Alternative Names:
CPC8, CYP2C8, CYPIIC8, Cytochrome P450 2C8

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only