Human DNM1L (Dynamin 1 Like Protein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynamin 1 Like Protein(DNM1L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dynamin 1 Like Protein(DNM1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynamin 1 Like Protein(DNM1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynamin 1 Like Protein(DNM1L) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity

Alternative Names:
DRP1, DLP1, DVLP, DYMPLE, HDYNIV, VPS1, Dynamin-related protein 1, Dnm1p/Vps1p-like protein, Dynamin family member proline-rich carboxyl-terminal domain less

Sample Type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only