Human EPCA2 (Early Prostate Cancer Antigen 2) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with EPCA2(Early Prostate Cancer Antigen 2) protein. Standards or samples are added to the appropriate microtiter plate wells then with EPCA2(Early Prostate Cancer Antigen 2) protein. Then add a biotin-conjugated EPCA2(Early Prostate Cancer Antigen 2) protein. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPCA2(Early Prostate Cancer Antigen 2) , biotin-conjugated protein and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPCA2(Early Prostate Cancer Antigen 2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal Transduction, Cancer

Alternative Names:
MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

Sample Type:
Serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 400 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only