Human EPHA5 (Ephrin Type A Receptor 5) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 5(EPHA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin Type A Receptor 5(EPHA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 5(EPHA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type A Receptor 5(EPHA5) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Neuro science,

Alternative Names:
TYRO4, CEK7, EHK1, HEK7, Brain-specific kinase, EPH homology kinase 1, EPH-like kinase 7

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only