Human FKBP1A (FK506 Binding Protein 1A) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1A(FKBP1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1A(FKBP1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1A(FKBP1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 1A(FKBP1A) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Immune molecule

Alternative Names:
FKBP-12, FKBP1, FKBP12C, PKC12, PKCI2, Calstabin-1, Rotamase, 12 kDa FK506-binding protein, Peptidyl-prolyl cis-trans isomerase FKBP1A, Immunophilin FKBP12

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only