Human FLNa (Filamin A Alpha) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha(FLNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin A Alpha(FLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha(FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha(FLNa) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Developmental science

Alternative Names:
ABP280, FLN-A, ABPX, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, Actin Binding Protein 280, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 120 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only