Human FLNb (Filamin B Beta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin B Beta(FLNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin B Beta(FLNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin B Beta(FLNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin B Beta(FLNb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Developmental science

Alternative Names:
ABP-278, AOI, FH1, FLN1L, LRS1, SCT, TABP, TAP, Actin Binding Protein 278, Actin-binding-like protein, Thyroid autoantigen, Filamin-3, Truncated actin-binding protein

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only