Human GALP (Galanin-like peptide) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GALP(Galanin-like peptide). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GALP(Galanin-like peptide). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GALP(Galanin-like peptide), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GALP(Galanin-like peptide) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Neuroscience, Cardiovascular, Developmental Biology

Alternative Names:
Galanin like peptide, Galanin like peptide precursor protein, Alarin splice variant, Alarin, GALP precursor protein

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 3.5h

Assay Length: For Research Use Only