Human GDF-15 ELISA Kit

INTENDED USE:
For the quantitative determination of human GDF-15 concentrations in serum, plasma or cell culture supernate samples

INTRODUCTION:
Growth differentiation factor 15 (GDF-15) belongs to transforming growth factor β family1. The biologically active form of GDF-15 is a 28 kDa disulphide linked homodimer2.It is also known as macrophage inhibiting cytokine 1 (MIC-1), placental transformation growth factor (PTGF-b), prostate derived factor (PDF),placental bone morphogenetic protein (PLAB), NSAID activated gene-1 (NAG-1) and PL741.

PRINCIPLE OF THE ASSAY:
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human GDF-15. Standards and samples are pipetted into the wells and any human GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.

SENSITIVITY: The lowest level of human GDF-15 that can be detected by this assay is 7.8 pg/mL.

PUBLICATIONS CITING THIS PRODUCT
Wu X, Xuan W, You L, Lian H, Li F, Zhang X, Chen Q, Sun K, Chen C, Xu M, Li Y, Yan L, Zhang X, Ren M. Associations of GDF-15 and GDF-15/adiponectin ratio with odds of type 2 diabetes in the Chinese population. Endocrine. 2021 Mar 12. doi: 10.1007/s12020-021-02632-1. Epub ahead of print. PMID: 33713014.