Human GIF (Gastric Intrinsic Factor) ELISA Kit
€0.00
In stock
SKU
ELK3212
Catalog Number: ELK3212
Reactivity: Human
Standard Range: 3.13-200 ng/mL
Sensitivity: 0.95 ng/mL
Information
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Reactivity: Human
Standard Range: 3.13-200 ng/mL
Sensitivity: 0.95 ng/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastric Intrinsic Factor(GIF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gastric Intrinsic Factor(GIF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastric Intrinsic Factor(GIF) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Endocrinology
Alternative Names:
TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis
Sample Type:
Serum, plasma and other biological fluids.
Assay Type: Competitive Inhibition
Standard: 200 ng/mL
Assay Length: 2h
Assay Length: For Research Use Only
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastric Intrinsic Factor(GIF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gastric Intrinsic Factor(GIF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastric Intrinsic Factor(GIF) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Endocrinology
Alternative Names:
TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis
Sample Type:
Serum, plasma and other biological fluids.
Assay Type: Competitive Inhibition
Standard: 200 ng/mL
Assay Length: 2h
Assay Length: For Research Use Only
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