Human GITR (Glucocorticoid Induced Tumor Necrosis Factor Receptor) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucocorticoid Induced Tumor Necrosis Factor Receptor(GITR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucocorticoid Induced Tumor Necrosis Factor Receptor(GITR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucocorticoid Induced Tumor Necrosis Factor Receptor(GITR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucocorticoid Induced Tumor Necrosis Factor Receptor(GITR) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Tumor immunity, Immune molecule

Alternative Names:
TNFRSF18, AITR, Tumor Necrosis Factor Receptor Superfamily 18, Glucocorticoid-induced TNFR-related protein, Activation-inducible TNFR family receptor

Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only