Human Hair Keratins polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
Keratin refers to a family of fibrous structural proteins. Keratin is the key structural material making up the outer layer of human skin. It is also the key structural component of hair and nails. Keratin monomers assemble into bundles to form intermediate filaments, which are tough and insoluble and form strong unmineralized tissues found in reptiles, birds, amphibians, and mammals. The only other biological matter known to approximate the toughness of keratinized tissue is chitin.

Isotype: Guinea pig Polyclonal (pan HK.1)

Immunogen:
Synthetic peptides common to human type I (acidic) andtype II (basic) hair (trichocytic) keratins K31-K40 (formerdesignation hHa1-hHa8, Ka35 and Ka36):LESEDCKLPSNP-C; and K81-K86 (former designationhHb1-hHb4): VNVCVSSSRGGVV-C, both coupled to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains most prominently human hair keratins K81-K86 (type II) and considerably weaker K31-K40 (type I) hair keratins present in the hair cortex, hair cuticle and tumors derived therefrom.

Reactivity: human, mouse

Applications: Suitable for immunofluorescence microscopy on frozen sections, immunohistochemistry on frozen sections and paraffin embedded material after microwave pretreatment, immunoblotting (Western)

Working DIlution: 1 : 100 for immunohistochemistry; 1 : 1,000 for Western blotting (ECL)

Storage: 2-8C

References:
- Langbein L, Schweizer J (2005) Intern Rev Cytology 243: 1-78