Human HBg1 (Hemoglobin Gamma 1) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Gamma 1(HBg1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Gamma 1(HBg1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Gamma 1(HBg1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Hematology

Alternative Names:
HB-G1, HBGA, HBGR, HSGGL1, Gamma-1-globin

Sample Type:
Serum, plasma, erythrocyte lysates.

Assay Type: Competitive Inhibition

Standard: 30000 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only