Human IFA (Intrinsic Factor Antibody)  ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFA(Intrinsic Factor Antibody). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IFA(Intrinsic Factor Antibody). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFA(Intrinsic Factor Antibody), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFA(Intrinsic Factor Antibody) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Metabolism

Alternative Names:
Gastric Intrinsic Factor, Gastric intrinsic factor (vitamin B synthesis), GIF, TCN3, TCM3

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 3.5h

Assay Length: For Research Use Only