Human IFNa/bR2 (Interferon Alpha/Beta Receptor 2) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha/Beta Receptor 2(IFNa/bR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha/Beta Receptor 2(IFNa/bR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha/Beta Receptor 2(IFNa/bR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Alpha/Beta Receptor 2(IFNa/bR2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity

Alternative Names:
IFNαR2, IFN-alpha-REC, IFN-R, IFNαBR, IFNαRB, Interferon(Alpha,Beta And Omega)Receptor Beta Chain, Interferon alpha binding protein, Type I interferon receptor 2

Sample Type:
Serum, plasma, urine and other biological fluids.

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only