Human IGF2R (Insulin Like Growth Factor 2 Receptor) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 Receptor(IGF2R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 Receptor(IGF2R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 Receptor(IGF2R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 Receptor(IGF2R) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Endocrinology

Alternative Names:
CD222, IGF2-R, CIMPR, M6P-R, MPRI, Cation-Independent Mannose-6 Phosphate Receptor, M6P/IGF2 receptor, 300 kDa mannose 6-phosphate receptor

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only