Human IgM polyclonal, anti-human

Background:
Immunoglobulin M (IgM) is the largest circulating antibody molecule in humans. It consists of a heavy chain (?-chain) and a light chain (κ- or λ-chain), as well as 5 base units and 10 binding sites, though it cannot bind all 10 simultaneously because of steric hindrance. IgM chain C refers to the constant region of the IgM heavy chain that is involved in immune regulation. IgM forms polymers by covalently linking multiple immunoglobulins together with disulfide bonds. It normally exists as a pentamer, but occasionally as a hexamer. Because of its polymeric nature, IgM has high avidity, and it is especially effective at complement activation. Due to its large size, IgM does not diffuse well, and it is found in the interstitium in very low amounts. IgM is mainly found in serum, however, because of the J chain, it is also important as a secretory immunoglobulin. IgM is the first immunoglobulin expressed by mature B cells, and it normally appears early in the course of an infection and does not reappear after further exposure.

Alternative Name:
IGHM, Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region, Immunoglobin heavy chain constant region mu, AGM1, Constant region of heavy chain of IgM, DKFZp686I15196, DKFZp686I15212, FLJ00385, Ig mu chain C region, IGHM, IgM heavy chain constant region, Immunoglobin heavy constant mu, Immunoglobulin mu, MGC104996, MGC52291, MU, VH,

Application Dilution:
WB:1:1,000-1:5,000
IHC:1:50-1:200

Specificity: Human IgM polyclonal antibody detects endogenous levels of Human IgM protein.

Immunogen:
recombinant protein

MW: 75 kDa

Swiss-Prot: P01871(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.