Human IL-17D ELISA Kit
€0.00
In stock
SKU
BEK1355
Catalog Number: BEK1355
Size(s): 96 tests/kit
Standard Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <15 pg/ml
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Size(s): 96 tests/kit
Standard Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <15 pg/ml
Request Information
Introduction:
IL-17D is the least characterized member of the IL-17 cytokine family (IL-17A-IL-17F). Identified in 2002, it exhibits distinct expression patterns and functions compared to other IL-17 cytokines. Unlike IL-17A/F (pro-inflammatory) or IL-17C (epithelial-derived), IL-17D is broadly expressed in non-immune tissues and plays roles in anti-tumor immunity, metabolic regulation, and viral defense.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-17D polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-17D polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-17D amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of IL-17D can be calculated.
Application:
For quantitative detection of IL-17D in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
IL-17D is the least characterized member of the IL-17 cytokine family (IL-17A-IL-17F). Identified in 2002, it exhibits distinct expression patterns and functions compared to other IL-17 cytokines. Unlike IL-17A/F (pro-inflammatory) or IL-17C (epithelial-derived), IL-17D is broadly expressed in non-immune tissues and plays roles in anti-tumor immunity, metabolic regulation, and viral defense.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-17D polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-17D polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-17D amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of IL-17D can be calculated.
Application:
For quantitative detection of IL-17D in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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