Human IL-25/ IL-17E ELISA Kit
€0.00
In stock
SKU
BEK1356
Catalog Number: BEK1356
Size(s): 96 tests/kit
Standard Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: <5 pg/ml
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Size(s): 96 tests/kit
Standard Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: <5 pg/ml
Request Information
Introduction:
Interleukin-25 (IL-25) – also known as interleukin-17E (IL-17E)– is a protein that in humans is encoded by the IL25 gene on chromosome 14. IL-25 was discovered in 2001 and is made up of 177 amino acids.IL-25 is a cytokine that belongs to the IL-17 cytokine family together with IL-17A (named also IL-17), IL-17B, IL-17C, IL-17D and IL-17F. This is why IL-25 has the alternative name IL-17E. All members have homologous amino acid sequence segments and spatially conserved cysteines. It is the IL-25 that differs from other members in its function and structure. IL-25 signals through a heterohexameric receptor complex containing IL-17RA and IL-17RB. In this complex, IL-25 forms a homodimer with IL-17RB, which then binds to IL-17RA.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-25 / IL-17E polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-25 / IL-17E polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-25 / IL-17E amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of IL-25 / IL-17E can be calculated.
Application:
For quantitative detection of IL-25 / IL-17E in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
Interleukin-25 (IL-25) – also known as interleukin-17E (IL-17E)– is a protein that in humans is encoded by the IL25 gene on chromosome 14. IL-25 was discovered in 2001 and is made up of 177 amino acids.IL-25 is a cytokine that belongs to the IL-17 cytokine family together with IL-17A (named also IL-17), IL-17B, IL-17C, IL-17D and IL-17F. This is why IL-25 has the alternative name IL-17E. All members have homologous amino acid sequence segments and spatially conserved cysteines. It is the IL-25 that differs from other members in its function and structure. IL-25 signals through a heterohexameric receptor complex containing IL-17RA and IL-17RB. In this complex, IL-25 forms a homodimer with IL-17RB, which then binds to IL-17RA.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-25 / IL-17E polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-25 / IL-17E polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-25 / IL-17E amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of IL-25 / IL-17E can be calculated.
Application:
For quantitative detection of IL-25 / IL-17E in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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