Human IL-32 ELISA Kit
€0.00
In stock
SKU
BEK1381
Catalog Number: BEK1381
Size(s): 96 tests/kit
Standard Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <4 pg/ml
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Size(s): 96 tests/kit
Standard Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <4 pg/ml
Request Information
Introduction:
Interleukin 32 (IL32) is proinflammatory cytokine that in humans is encoded by the IL32 gene.Interleukin 32 can be found in higher mammals but not in rodents. It is mainly expressed intracellularly and the protein has nine different isoforms, because the pre-mRNA can be alternatively spliced.The most active and studied isoform is IL-32γ. It was first reported in 2005,although the IL-32 gene was first described in 1992.It does not belong to any cytokine family because there is almost no homology with other cytokines.mRNA of IL-32 is mostly expressed in immune cells but also can be expressed in other tissues such as spleen, thymus, lung, small intestine, colon, prostate, heart, placenta, liver, muscle, kidney, pancreas and brain.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-32 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-32 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-32 amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of IL-32 can be calculated.
Application:
For quantitative detection of IL-32 in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
Interleukin 32 (IL32) is proinflammatory cytokine that in humans is encoded by the IL32 gene.Interleukin 32 can be found in higher mammals but not in rodents. It is mainly expressed intracellularly and the protein has nine different isoforms, because the pre-mRNA can be alternatively spliced.The most active and studied isoform is IL-32γ. It was first reported in 2005,although the IL-32 gene was first described in 1992.It does not belong to any cytokine family because there is almost no homology with other cytokines.mRNA of IL-32 is mostly expressed in immune cells but also can be expressed in other tissues such as spleen, thymus, lung, small intestine, colon, prostate, heart, placenta, liver, muscle, kidney, pancreas and brain.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-32 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-32 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-32 amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of IL-32 can be calculated.
Application:
For quantitative detection of IL-32 in human serum, plasma, body fluids, tissue lysatesor cellculture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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