Human IL-5 (His-tag) recombinant active protein
€550.00
In stock
SKU
BT-CI59
Background Information: IL-5 is expressed in eosinophils, NK cells, TC2 CD8+ T cells, mast cells, CD45+ CD4+ T cells, gamma delta T cells and IL-1 beta activated endothelial cells. IL-5 acts as a growth and differentiation factor for both B cells and eosinophils. Relative to B cells, IL-5 appears to induce the differentiation of activated conventional B-2 cells into Ig-secreting cells. In addition, it induces the growth of B-1 progenitors as well as IgM production by B-1 cells.IL-5 appears to perform a number of functions on eosinophils. These include the down modulation of Mac-1,the upregulation of receptors for IgA and IgG,the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release.IL-5 also promotes the growth and differentiation of eosinophils.
Target Name:
Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5
Source:
Recombinant Human IL-5 (accession number P05113) (Ile20-Ser134)with a 6His tag at the C-terminus is expressed in HEK293 cells.
Molecular Weight:
The 129 amino acid protein has the predicted molecular weight of 14.2kDa. The protein migrates at approximately 20kDa on SDS-PAGE with DTT-reduced condition
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Bioactivity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/ml.
Endotoxin level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Target Name:
Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5
Source:
Recombinant Human IL-5 (accession number P05113) (Ile20-Ser134)with a 6His tag at the C-terminus is expressed in HEK293 cells.
Molecular Weight:
The 129 amino acid protein has the predicted molecular weight of 14.2kDa. The protein migrates at approximately 20kDa on SDS-PAGE with DTT-reduced condition
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Bioactivity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/ml.
Endotoxin level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
| Is Featured? | No |
|---|
Write Your Own Review