Human IL1e (Interleukin 1 Epsilon) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Epsilon(IL1e). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Epsilon(IL1e). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Epsilon(IL1e), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Epsilon(IL1e) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine

Alternative Names:
IL1F6, IL36a, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), Interleukin 36, Alpha, Interleukin 1 Family, Member 6

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Type: Sandwich

Standard: 500 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only