Human INPP4B (Inositol Polyphosphate-4-Phosphatase Type II 105kDa) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-4-Phosphatase Type II 105kDa(INPP4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inositol Polyphosphate-4-Phosphatase Type II 105kDa(INPP4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-4-Phosphatase Type II 105kDa(INPP4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Polyphosphate-4-Phosphatase Type II 105kDa(INPP4B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
Type II inositol 3,4-bisphosphate 4-phosphatase

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only