Human Interleukin-12(p70), IL-12(p70) HiLA Kit
€0.00
In stock
SKU
BHK1011
Introduction:
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells,macrophages, neutrophils, helper T cells and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12.IL12 is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis. IL12A is composed of a bundle of four alpha helices. IL12B has three beta sheet domains.
Principle of the Assay:
Incubate the Fconjugated anti-IL-12(p70) antibody 1, biotin conjugated anti-IL-12(p70) antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-12(p70)in the sample is calculated through mathe matical fitting. In the absence of humanIL-12(p70)in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human IL-12(p70) in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells,macrophages, neutrophils, helper T cells and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12.IL12 is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis. IL12A is composed of a bundle of four alpha helices. IL12B has three beta sheet domains.
Principle of the Assay:
Incubate the Fconjugated anti-IL-12(p70) antibody 1, biotin conjugated anti-IL-12(p70) antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-12(p70)in the sample is calculated through mathe matical fitting. In the absence of humanIL-12(p70)in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human IL-12(p70) in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
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