Human Interleukin-17F, IL-17F HiLA Kit
€0.00
In stock
SKU
BHK1013
Introduction:
Interleukin 17F (IL-17F) is signaling protein that is in human which is encoded by the IL17F gene and is considered a pro-inflammatory cytokine. This protein belongs to the interleukin 17 family and is mainly produced by the T helper 17 cells after their stimulation with interleukin 23. However, IL-17F can be also produced by a wide range of cell types, including innate immune cells and epithelial cells.
Principle of the Assay:
Incubate the Fconjugated anti-IL-17F antibody 1, biotin conjugated anti-IL-17F antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-17Fin the sample is calculated through mathe matical fitting. In the absence of humanIL-17Fin the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human IL-17F in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
Interleukin 17F (IL-17F) is signaling protein that is in human which is encoded by the IL17F gene and is considered a pro-inflammatory cytokine. This protein belongs to the interleukin 17 family and is mainly produced by the T helper 17 cells after their stimulation with interleukin 23. However, IL-17F can be also produced by a wide range of cell types, including innate immune cells and epithelial cells.
Principle of the Assay:
Incubate the Fconjugated anti-IL-17F antibody 1, biotin conjugated anti-IL-17F antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-17Fin the sample is calculated through mathe matical fitting. In the absence of humanIL-17Fin the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human IL-17F in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
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