Human Interleukin-22, IL-22 HiLA Kit
€0.00
In stock
SKU
BHK1014
Introduction:
Interleukin-22 (IL-22) is a protein that in humans is encoded by the IL22 gene.IL-22 is an α-helical cytokine. IL-22 binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits. IL-22R is expressed on tissue cells, and it is absent on immune cells. Crystallization is possible if the N-linked glycosylation sites are removed in mutants of IL-22 bound with high-affinity cell-surface receptor sIL-22R1. The crystallographic asymmetric unit contained two IL-22-sIL-22R1 complexes.
Principle of the Assay:
Incubate the Fconjugated anti-IL-22 antibody 1, biotin conjugated anti-IL-22 antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-22in the sample is calculated through mathe matical fitting. In the absence of humanIL-22in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human IL-22 in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
Interleukin-22 (IL-22) is a protein that in humans is encoded by the IL22 gene.IL-22 is an α-helical cytokine. IL-22 binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits. IL-22R is expressed on tissue cells, and it is absent on immune cells. Crystallization is possible if the N-linked glycosylation sites are removed in mutants of IL-22 bound with high-affinity cell-surface receptor sIL-22R1. The crystallographic asymmetric unit contained two IL-22-sIL-22R1 complexes.
Principle of the Assay:
Incubate the Fconjugated anti-IL-22 antibody 1, biotin conjugated anti-IL-22 antibody 2,acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex ( antibody 1-sample- antibody 2). the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-22in the sample is calculated through mathe matical fitting. In the absence of humanIL-22in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Application:
For quantitative detection of human IL-22 in buffer solution, serum, cell culture supernatant.
Storage and Expiration:
Store at 2-8℃ for 6 months.
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