Human IS (serum indoxyl sulfate) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to serum indoxyl sulfate(IS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to serum indoxyl sulfate(IS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain serum indoxyl sulfate(IS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of serum indoxyl sulfate(IS) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Kidney diseases

Alternative Names:
IS, indoxyl sulfate, serum indoxyl sulfate

Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 2000 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only