Human KLK12 (Kallikrein 12) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 12(KLK12) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 12(KLK12). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 12(KLK12) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
KLK-L5, KLKL5, Kallikrein-Related Peptidase 12, Kallikrein-like protein 5

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Competitive Inhibition

Standard: 1000 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only