Human LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-1(LAB7-1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-1(LAB7-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-1(LAB7-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Activation Antigen B7-1(LAB7-1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: CD & Adhesion molecule, Tumor immunity, Infection immunity, Immune molecule, Autoimmunity

Alternative Names:
CD80, CD28LG, CD28LG1, BB1, B7-1 Antigen, T-lymphocyte activation antigen CD80, CTLA-4 counter-receptor B7.1

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only