Human MAN2B1 (Mannosidase Alpha Class 2B Member 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1(MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1(MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1(MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 2B Member 1(MAN2B1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
MANB, LAMAN, Lysosomal alpha-mannosidase, Lysosomal acid alpha-mannosidase, Mannosidase alpha-B

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only