Human MCP-3 ELISA Kit
€0.00
In stock
SKU
BEK1145
Introduction:
Monocyte-specific chemokine 3 (MCP3), also called chemokine (C-C motif) ligand 7 (CCL7), is asmall cytokine known as a chemokinethatbelongs to the C-C chemokine family.It is produced by certain tumor cell lines and by macrophages.This chemokine is located on chromosome 17 in human s, in a large cluster containing many other CC chemokinesand is most closely related to CCL2 (previously called MCP1).Itwas identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation.
Principle of the Assay:
This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MCP-3 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MCP-3antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-3 can be calculated.
Application:
For quantitative detection of MCP-3 in human serum,plasma, urine, cell culture supernatant or tissue samples.
Storage and Expiration:
Store at 2-8℃ for 6 months.
Monocyte-specific chemokine 3 (MCP3), also called chemokine (C-C motif) ligand 7 (CCL7), is asmall cytokine known as a chemokinethatbelongs to the C-C chemokine family.It is produced by certain tumor cell lines and by macrophages.This chemokine is located on chromosome 17 in human s, in a large cluster containing many other CC chemokinesand is most closely related to CCL2 (previously called MCP1).Itwas identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation.
Principle of the Assay:
This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MCP-3 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MCP-3antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-3 can be calculated.
Application:
For quantitative detection of MCP-3 in human serum,plasma, urine, cell culture supernatant or tissue samples.
Storage and Expiration:
Store at 2-8℃ for 6 months.
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