Human MEP1b (Meprin A Beta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Beta(MEP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meprin A Beta(MEP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meprin A Beta(MEP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meprin A Beta(MEP1b) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway

Alternative Names:
PPH Beta, Endopeptidase-2, Meprin B, PABA Peptide Hydrolase, N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Beta

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only