Human MGAT5 (Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase(MGAT5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase(MGAT5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase(MGAT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase(MGAT5) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
GNT-V, GGNT5, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only