Human MHCF (Major Histocompatibility Complex Class I F) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I F(MHCF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I F(MHCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I F(MHCF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I F(MHCF) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Tumor immunity, Infection immunity, Immune molecule, Developmental science

Alternative Names:
CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only