Human MICA (MHC class I chain-related protein A) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MICA(MHC class I chain-related protein A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MICA(MHC class I chain-related protein A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MICA(MHC class I chain-related protein A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MICA(MHC class I chain-related protein A) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Immunology

Alternative Names:
major histocompatibility complex class I chain-related gene A, MHC class I chain-related protein B, MHC class I polypeptide related sequence A, MHC class I polypeptide related sequence B, MIC-A

Sample Type:
serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only