Human MT-RNR2 (Putative humanin peptide) ELISA kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Putative humanin peptide(MT-RNR2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Putative humanin peptide(MT-RNR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Putative humanin peptide(MT-RNR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Putative humanin peptide(MT-RNR2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Others

Alternative Names:
MT-RNR2, HN , Humanin , Humanin mitochondrial , HNM

Sample Type:
serum, plasma, tissue homogenates, cell lysates

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only