Human NCCT (Na-Cl Cotransporter) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na-Cl Cotransporter(NCCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Na-Cl Cotransporter(NCCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Na-Cl Cotransporter(NCCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Na-Cl Cotransporter(NCCT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
SLC12A3, TSC, NCC, Solute Carrier Family 12 Member 3,Sodium/Chloride Transporters, Thiazide-sensitive sodium-chloride cotransporter, Na-Cl symporter

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only