Human NFYC (Nuclear Transcription Factor Y Gamma) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Transcription Factor Y Gamma(NFYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Transcription Factor Y Gamma(NFYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Transcription Factor Y Gamma(NFYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Transcription Factor Y Gamma(NFYC) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Neuro science,

Alternative Names:
CBF-C, NFYg, CBFC, H1TF2A, HAP5, HSM, NF-YC, hCBF-C, CAAT box DNA-binding protein subunit C, Nuclear transcription factor Y subunit C, Transactivator HSM-1/2

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only