Human NKX3-1 (NK3 Homeobox Protein 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NK3 Homeobox Protein 1(NKX3-1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NK3 Homeobox Protein 1(NKX3-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NK3 Homeobox Protein 1(NKX3-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NK3 Homeobox Protein 1(NKX3-1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
BAPX2, NKX3, NKX3.1, NKX3A, NK3 transcription factor related, locus 1, Homeobox protein NK-3 homolog A

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only