Human NONO (Non POU Domain Containing Octamer Binding Protein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein(NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein(NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein(NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non POU Domain Containing Octamer Binding Protein(NONO) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
P54, NMT55, NRB54, P54NRB, Nuclear RNA-Binding Protein 54-kD, 54 kDa nuclear RNA- and DNA-binding protein, DNA-binding p52/p100 complex, 52 kDa subunit

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only