Human NRF1 (Nuclear respiratory factor 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NRF1(Nuclear respiratory factor 1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NRF1(Nuclear respiratory factor 1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NRF1(Nuclear respiratory factor 1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NRF1(Nuclear respiratory factor 1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Epigenetics and Nuclear Signaling, Metabolism

Alternative Names:
alpha pal, alpha palindromic binding protein, locus control region factor 1, NFE2 related factor 1, NRF-1, Nrf1

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only