Human OB (Obestatin) ELISA Kit
Catalog Number: ACE-E1715
Size: 96 tests/kit
Detection Range: 0.78—50 ng/mL
Sensitivity: 0.47 ng/mL
Datasheet
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Size: 96 tests/kit
Detection Range: 0.78—50 ng/mL
Sensitivity: 0.47 ng/mL
Datasheet
Request Information
Price on request
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You are enquiring about Human OB (Obestatin) ELISA Kit
Description:
This ELISA kit applies to the in vitro quantitative determination of Human OB concentrations in serum, plasma and other biological fluids.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human OB. During the reaction, Human OB in the sample or standard competes with a fixed amount of Human OB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human OB. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human OB in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Human
Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
This ELISA kit applies to the in vitro quantitative determination of Human OB concentrations in serum, plasma and other biological fluids.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human OB. During the reaction, Human OB in the sample or standard competes with a fixed amount of Human OB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human OB. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human OB in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Human
Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
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