Human p-cresol ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p-cresol. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Neuro science,

Alternative Names:
4-Hydroxytoluene, 4-Methylphenol, p-Cresol 98+ %, PARA-CRESOL, 4-cresol, para cresol

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 200 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only