Human PCI (Protein C Inhibitor) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C Inhibitor(PCI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein C Inhibitor(PCI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C Inhibitor(PCI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C Inhibitor(PCI) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity

Alternative Names:
SERPINA5, PAI3, PLANH3, PROCI, Serpin Peptidase Inhibitor Clade A 5, Plasma Serine Protease Inhibitor, Plasminogen Activator Inhibitor 3, Acrosomal serine protease inhibitor

Sample Type:
plasma

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only