Human PDZRN3 (PDZ domain containing ring finger 3) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to soluble Triggering Receptor Expressed on PDZRN3(PDZ domain containing ring finger 3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to soluble Triggering Receptor Expressed on PDZRN3(PDZ domain containing ring finger 3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain soluble Triggering Receptor Expressed on PDZRN3(PDZ domain containing ring finger 3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of soluble Triggering Receptor Expressed on PDZRN3(PDZ domain containing ring finger 3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Developmental Biology

Alternative Names:
E3 ubiquitin-protein ligase PDZRN3, KIAA1095, Ligand of Numb protein X 3, likely ortholog of mouse semaF cytoplasmic domain associated protein 3, LNX3, PDZ domain containing ring finger 3, PDZ domain-containing RING finger protein 3, Pdzrn3, Protein SEMACAP3, PZRN3_HUMAN, SEMACAP3, Semaphorin cytoplasmic domain associated protein, Semaphorin cytoplasmic domain-associated protein 3, SEMCAP3

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only