Human PLCg1 (Phospholipase C Gamma 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Gamma 1(PLCg1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase C Gamma 1(PLCg1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Gamma 1(PLCg1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Gamma 1(PLCg1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Tumor immunity

Alternative Names:
NCKAP3, PLC-G1, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, Phosphoinositide phospholipase C-gamma-1

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only